PDB ID or protein name

1dsy » Protein kinase C alpha, complex with phosphatidyl serine

1dsy » Protein kinase C alpha, complex with phosphatidyl serine
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Topology in Eukaryotic plasma membrane
Topologyextracellular side
cytoplasmic side
1dsy » Protein kinase C alpha, complex with phosphatidyl serine
Depth 0.9 ± 1.5 Å
Tilt Angle 18 ± 16°
ΔGtransfer -3.0 kcal/mol
Links to 1dsy PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology cytoplasmic
Resolution 2.60 Å
Other PDB entries representing this structure 3gpe, 3rdj, 3rdl, 3twy, 4l1l
Number of subunits 1
Experimental Verification for 1dsy » Protein kinase C alpha, complex with phosphatidyl serine
Side-chains of P188, N189, and T250 penetrate the hydrocarbon core. Residues in positions 188 and 250 were not spin-labeled. Residues N189, R249 and R252 were shown to be the closest to the membrane (positive depth parameters after substitutions of the corresponding residues by spin-labeled cysteines) (Kohout et al. 2003, Table 3). Two calcium ions are located at the distances of 5.3 and 5.9 A beyond the hydrophobic boundary, i.e. 0.3 and 0.9 A, respectively, beyond the level of bulk lipid phosphates. This is consistent with EPR studies (Malmberg and Falke 2005). Maximal membrane binding affinity is -6.7 kcal/mol (Kohout et al. 2003), -10.8 kcal/mol (Stahelin and Cho 2001), or -12.8 kcal/mol (Stahelin et al. 2003). This is higher than the calculated transfer energy (-0.6 kcal/mol without the ligand) due to specific binding of the phosphatidyl serine.
4 references
Kohout SC, Corbalan-Garcia S, Gomez-Fernandez JC, and Falke JJ (2003) C2 domain of protein kinase C alpha: Elucidation of the membrane docking surface by site-directed fluorescence and spin labeling. Biochemistry42: 1254-1265. PubMed
Malmberg NJ, Falke JJ. 2005. Use of EPR power saturation to analyze the membrane-docking geometries of peripheral proteins: applications to C2 domains. Annu Rev Biophys Biomol Struct. 34: 71-90. PubMed
Stahelin RV, Cho W. 2001. Roles of calcium ions in the membrane binding of C2 domains. Biochem J. 359:679-85. PubMed
Stahelin RV, Rafter JD, Das S, Cho W. 2003. The molecular basis of differential subcellular localization of C2 domains of protein kinase C-alpha and group IVa cytosolic phospholipase A2. J Biol Chem. 278:12452-60. PubMed