1dvp » Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs)
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Topology in Endosome membrane
Download Coordinates
Topology in Endosome membrane
 | lumenal side |
| cytoplasmic side |
- Type: 2. Monotopic/peripheral (4 classes)
- Class: 2.1. All alpha monotopic/peripheral (31 superfamilies)
- Superfamily: 2.1.15. ENTH/VHS domain (3 families)
- Family: 2.1.15.02. VHS domain (1 protein)
- Species: Drosophila melanogaster (4 proteins)
- Localization: Endosome membrane (12 proteins)
| 1dvp » Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) | |
|---|---|
| Depth | 2.6 ± 0.3 Å |
| Tilt Angle | 90 ± 5° |
| ΔGtransfer | -6.5 kcal/mol |
| Links to 1dvp | PDB Sum, SCOP, MSD, OCA, MMDB, Dali |
| Topology | cytoplasmic |
| Resolution | 2.00 Å |
| Related PDB Sum entries | none |
| Number of subunits | 2 |
| Experimental Verification for 1dvp » Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) |
|---|
| Consistent with membrane-binding mode proposed by Mao et al. (2000) and Diraviyam et al. (2003). Monomeric FYVE domain has a partialy different orientation (Blatner et al. 2004). |
| 3 references |
|---|
| Blatner NR, Stahelin RV, Diraviyam K, Hawkins PT, Hong W, Murray D, Cho W. 2004. The molecular basis of the differential subcellular localization of FYVE domains. J Biol Chem. 279:53818-27. PubMed |
| Diraviyam K, Stahelin RV, Cho W, Murray D. 2003. Computer modeling of the membrane interaction of FYVE domains. J Mol Biol. 328:721-36. PubMed |
| Mao Y, Nickitenko A, Duan X, Lloyd TE, Wu MN, Bellen H, Quiocho FA. 2000. Crystal structure of the VHS and FYVE tandem domains of Hrs, a protein involved in membrane trafficking and signal transduction. Cell. 100:447-56. PubMed |
| Comments on 1dvp » Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) |
|---|
| Essential role in endosome membrane invagination and formation of multivesicular bodies, MVBs. Each monomeric unit in the dimer includes a VHS and a FYVE domain. Additional membrane-anchoring elements: inositol lipids that are specifically bound to FYVE domains, consistent with the calculated membrane-association mode. |
