1gmi » C2 domain of protein kinase C epsilon
3D view in Chime, Jmol or Webmol
Download Coordinates
Topology in Eukaryotic plasma membrane
Download Coordinates
Topology in Eukaryotic plasma membrane
 | extracellular side |
| cytoplasmic side |
- Type: 2. Monotopic/peripheral (4 classes)
- Class: 2.2. All beta monotopic/peripheral (42 superfamilies)
- Superfamily: 2.2.11. C2 domain (2 families)
- Family: 2.2.11.01. PLC-like (P variant) (7 proteins)
- Species: Rattus rattus (2 proteins)
- Localization: Eukaryotic plasma membrane (198 proteins)
| 1gmi » C2 domain of protein kinase C epsilon | |
|---|---|
| Depth | 2.0 ± 1.1 Å |
| Tilt Angle | 41 ± 10° |
| ΔGtransfer | -5.1 kcal/mol |
| Links to 1gmi | PDB Sum, SCOP, MSD, OCA, MMDB, Dali |
| Topology | cytoplasmic |
| Resolution | 1.7 Å |
| Related PDB Sum entries | none |
| Number of subunits | 1 |
| Experimental Verification for 1gmi » C2 domain of protein kinase C epsilon |
|---|
| Residue I89 and partially V29 and P31 penetrate the bilayer core. According to muatgenesis studies, the most important residue for membrane binding is I89 (Corbalan-Garcia et al. 2003). The binding is also affected by replacing Y91 and in the triple mutant W23A/R26A/R32A. These residues are in the membrane interfacial region in the calculated orientation. |
| 1 reference |
|---|
| Corbalan-Garcia S, Sanchez-Carrillo S, Garcia-Garcia J, Gomez-Fernandez JC. 2003. Characterization of the membrane binding mode of the C2 domain of PKC epsilon. Biochemistry. 42:11661-8. PubMed |
