PDB ID or protein name

1grm » Gramicidin A, head-to-head dimer, right-handed

1grm » Gramicidin A, head-to-head dimer, right-handed
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Topology in Secreted
Topologyout side
in side
1grm » Gramicidin A, head-to-head dimer, right-handed
Hydrophobic Thickness or Depth 23.2 ± 4.0 Å
Tilt Angle 7 ± 9°
ΔGtransfer -28.1 kcal/mol
Links to 1grm PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology undef
Resolution NMR
Other PDB entries representing this structure 1jno, 1mag, 1ng8, 1nrm, 1nru, 1nt5, 1nt6
Number of TM Secondary Structures 1
2 transmembrane subunits
A - Tilt: 27° - Segments: 1(2-14)
B - Tilt: 27° - Segments: 1(2-14)
Experimental Verification for 1grm » Gramicidin A, head-to-head dimer, right-handed
Structure in SDS micelles. Results are consistent with the experimental hydrophobic thickness of gramicidin A dimer (Elliott et al. 1983, Harroun et al. 1999) and with its nearly perpendicular arrangement to the membrane, as determined by solid state NMR and IR spectroscopy (Nabedryk et al. 1982, Andronesi et al. 2004, Andersen et al. 2005). However, ATR FTIR spectroscopy gives a large dynamically averaged tilt angle (Kota et al. 2004).
6 references
Andersen OS, Koeppe RE, and Roux B (2005) Gramicidin channels. IEEE Transact. Nanobiosci. 4: 10-20. PubMed
Andronesi OC, Pfeifer JR, Al-Momani L, Ozdirekcan S, Rijkers DTS, Angerstein B, Luca S, Koert U, Killian JA, and Baldus M (2004) Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR. J. Biomol. NMR 30: 253-265 PubMed
Elliott JR, Needham D, Dilger JP, and Haydon DA (1983) The effects of bilayer thickness and tension on gramicidin single-channel lifetime. Biochim. Biophys. Acta 735: 95-103. PubMed
Harroun TA, Heller WT, Weiss TM, Yang L, and Huang HW (1999) Experimental evidence for hydrophobic matching and membrane-mediated interactions in lipid bilayers containing gramicidin. Biophys. J. 76: 937-945. PubMed
Kota Z, Pali T, and Marsh D (2004) Orientation and lipid-peptide interactions of gramicidin A in lipid membranes: Polarized attenuated total reflection infrared spectroscopy and spin-label electron spin resonance. Biophys. J. 86: 1521-1531. PubMed
Nabedryk E, Gingold MP, and Breton J (1982) Orientation of gramicidin A transmembrane channel. Infrared dichroism study of gramicidin in vesicles. Biophys J. 38: 243-249. PubMed