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1gzm » Rhodopsin

• Type: 1. Transmembrane (2 classes)
• Class: 1.1. Alpha-helical transmembrane (54 superfamilies)
• Superfamily: 1.1.01. Rhodopsin-like proteins (2 families)
• Family: 1.1.01.02. G-protein coupled receptors (15 proteins)
• Species: Bos taurus (38 proteins)
• Localization: Eukaryotic plasma membrane (195 proteins)

1gzm » Rhodopsin
Hydrophobic Thickness	32.4 ± 1.7 Å
Tilt Angle	8 ± 3°
ΔGtransfer	-91.3 kcal/mol
Links to 1gzm	PDB Sum, SCOP, MSD, OCA, MMDB, Dali
Topology	subunit A (N-terminus extracellular)
Resolution	2.70 Å
Related PDB Sum entries	1f88, 1hzx, 1jfp, 1l9h, 1ln6, 1u19, 2g87, 2hpy, 2i35, 2i36, 2i37, 2iqk, 2ped, 3c9l, 3c9m, 3cap
Number of TM Secondary Structures	7

1 transmembrane subunit
A - Tilt: 8° - Segments: 1(38-63), 2(72-96), 3(109-133), 4(153-172), 5(202-224), 6(253-274), 7(286-309)

Experimental Verification for 1gzm » Rhodopsin
Results are consitent with X-ray scattering (Blaurock and Wilkins 1972), chemical modification (Barclay and Findlay 1984, Davison and Findlay 1986a,b) and electron microscopy (Krebs et al. 2003) studies. Hydrophobic boundaries of rhodopsin expand when calculated with detergent parameters (Figure 4), in agreement with results of Hubbell et al. (2003).

6 references
Barclay P, and Findlay JBC (1984) Labelling of the cytoplasmic domains of ovine rhodopsin with hydrophilic chemical probes. Biochem. J. 220: 75-84. PubMed
Blaurock AE, and Wilkins MHF (1972) Structure of retinal photoreceptor membranes. Nature 236: 313-314. PubMed
Davison MD, and Findlay JBC (1986) Identification of the sites in opsin modified by photoactivated azido[125]iodobenzene. Biochem. J. 236: 389-395. PubMed
Davison MD, and Findlay JBC (1986) Modification of ovine opsin with the photosensitive hydrophobic probe 1-azido-4-[125]-iodobenzene. Biochem. J. 234: 413-420. PubMed
Hubbell WL, Altenbach C, Hubbell CM, and Khorana HG (2003) Rhodopsin structure, dynamics, and activation: A perspective from crystallography, site-directed spin labeling, sulfhydryl reactivity, and disulfide cross-linking. Adv. Prot. Chem. 63: 243-290. PubMed
Krebs A, Edwards PC, Villa C, Li JD, and Schertler GFX (2003) The three-dimensional structure of bovine rhodopsin determined by electron cryomicroscopy. J. Biol. Chem. 278: 50217-50225. PubMed

Copyright © 2005- Andrei Lomize, Mikhail Lomize and Irina Pogozheva
University of Michigan, Ann Arbor, MI 48109 USA