PDB ID or protein name

1gzm » Rhodopsin, inactive, complex with inverse agonist, 11-cis retinal

1gzm » Rhodopsin, inactive, complex with inverse agonist, 11-cis retinal
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Topology in Eukaryotic plasma membrane
Topologyextracellular side
cytoplasmic side
1gzm » Rhodopsin, inactive, complex with inverse agonist, 11-cis retinal
Hydrophobic Thickness 32.2 ± 1.5 Å
Tilt Angle 11 ± 1°
ΔGtransfer -75.6 kcal/mol
Links to 1gzm PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology subunit A (N-terminus extracellular)
Resolution 2.70 Å
Other PDB entries representing this structure 2i35, 2j4y, 3c9l, 3c9m
Number of TM Secondary Structures 7
1 transmembrane subunit
A - Tilt: 11° - Segments: 1(35-63), 2(74-99), 3(109-133), 4(153-172), 5(202-224), 6(253-276), 7(286-308)
Experimental Verification for 1gzm » Rhodopsin, inactive, complex with inverse agonist, 11-cis retinal
Results are consitent with X-ray scattering (Blaurock and Wilkins 1972), chemical modification (Barclay and Findlay 1984, Davison and Findlay 1986a,b) and electron microscopy (Krebs et al. 2003) studies. Hydrophobic boundaries of rhodopsin expand when calculated with detergent parameters (Figure 4), in agreement with results of Hubbell et al. (2003).
6 references
Barclay P, and Findlay JBC (1984) Labelling of the cytoplasmic domains of ovine rhodopsin with hydrophilic chemical probes. Biochem. J. 220: 75-84. PubMed
Blaurock AE, and Wilkins MHF (1972) Structure of retinal photoreceptor membranes. Nature 236: 313-314. PubMed
Davison MD, and Findlay JBC (1986) Identification of the sites in opsin modified by photoactivated azido[125]iodobenzene. Biochem. J. 236: 389-395. PubMed
Davison MD, and Findlay JBC (1986) Modification of ovine opsin with the photosensitive hydrophobic probe 1-azido-4-[125]-iodobenzene. Biochem. J. 234: 413-420. PubMed
Hubbell WL, Altenbach C, Hubbell CM, and Khorana HG (2003) Rhodopsin structure, dynamics, and activation: A perspective from crystallography, site-directed spin labeling, sulfhydryl reactivity, and disulfide cross-linking. Adv. Prot. Chem. 63: 243-290. PubMed
Krebs A, Edwards PC, Villa C, Li JD, and Schertler GFX (2003) The three-dimensional structure of bovine rhodopsin determined by electron cryomicroscopy. J. Biol. Chem. 278: 50217-50225. PubMed
Comments on 1gzm » Rhodopsin, inactive, complex with inverse agonist, 11-cis retinal
Structures of intermediate states: bathorodopsin (2g87) and lumirhodopsin (2hpy). Complex with 9-cis retinal: 2ped.