PDB ID or protein name

1hce » Hisactophilin-1

1hce » Hisactophilin-1
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Topology in Eukaryotic plasma membrane
Topologyextracellular side
cytoplasmic side
1hce » Hisactophilin-1
Depth 3.5 ± 1.1 Å
Tilt Angle 60 ± 15°
ΔGtransfer -1.6 kcal/mol
Links to 1hce PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology cytoplasmic
Resolution NMR
Other PDB entries representing this structure 1hcd
Number of subunits 1
Experimental Verification for 1hce » Hisactophilin-1
Contains N-myristoyl Gly 1 residue in vivo (it is replaced by Met in the PDB entry). Membrane-binding affinity of the myristoylated protein at pH 6: -8.1 kcal/mol (2.5 kcal/mol comes from electrostatic interactions of seven charged histidines with membrane, and 5.6 kcal/mol comes from transfer of a myristoyl group) (Hanakan et al. 1996).
1 reference
Hanakam, F., Gerisch, G., Lotz, S., Alt, T., and Seelig, A. 1996. Binding of hisactophilin I and II to lipid membranes is controlled by a pH-dependent myristoyl-histidine switch. Biochemistry. 35:11036-11044 PubMed
Comments on 1hce » Hisactophilin-1
May act as an intracellular pH sensor that links chemotactic signals to responses in the microfilament system of the cells by nucleating actin polymerization or stabilizing the filaments.