1hxx » Porin OmpF
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Topology in Bacterial gram-negative outer membrane
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Topology in Bacterial gram-negative outer membrane
 | extracellular side |
| periplasmic side |
- Type: 1. Transmembrane (2 classes)
- Class: 1.2. Beta-barrel transmembrane (19 superfamilies)
- Superfamily: 1.2.09. Trimeric porins (n=16,S=20) (3 families)
- Family: 1.2.09.01. General Bacterial Porin (GBP) (7 proteins) 1.B.1 (TCDB)
- Species: Escherichia coli (93 proteins)
- Localization: Bacterial gram-negative outer membrane (71 proteins)
| 1hxx » Porin OmpF | |
|---|---|
| Hydrophobic Thickness | 24.2 ± 0.8 Å |
| Tilt Angle | 0 ± 1° |
| ΔGtransfer | -147.6 kcal/mol |
| Links to 1hxx | PDB Sum, SCOP, MSD, OCA, MMDB, Dali |
| Topology | subunit A (N-terminus periplasmic) |
| Resolution | 2.20 Å |
| Related PDB Sum entries | 1bt9, 1gfm, 1gfn, 1gfo, 1gfp, 1gfq, 1hxt, 1hxu, 1mpf, 1opf, 2omf, 2zfg, 2zld, 3fyx, 3hw9, 3hwb |
| Number of TM Secondary Structures | 48 |
| 3 transmembrane subunits |
|---|
| A - Tilt: 3° - Segments: 1(11-23), 2(39-48), 3(55-66), 4(79-90), 5(94-99), 6(135-142), 7(151-158), 8(173-182), 9(185-192), 10(214-222), 11(225-232), 12(257-265), 13(269-276), 14(294-302), 15(307-315), 16(332-339) |
| B - Tilt: 3° - Segments: 1(11-23), 2(39-48), 3(55-66), 4(79-90), 5(94-99), 6(135-142), 7(151-158), 8(173-182), 9(185-192), 10(214-222), 11(225-232), 12(257-265), 13(269-276), 14(294-302), 15(307-315), 16(332-339) |
| C - Tilt: 3° - Segments: 1(11-23), 2(39-48), 3(55-66), 4(79-90), 5(94-99), 6(135-142), 7(151-158), 8(173-182), 9(185-192), 10(214-222), 11(225-232), 12(257-265), 13(269-276), 14(294-302), 15(307-315), 16(332-339) |
| Experimental Verification for 1hxx » Porin OmpF |
|---|
| Results are consistent with experimental hydrophobic thickness of OmpF porin (Keeffe et al. 2000) and hydrophobic boundaries as determined by neutron diffraction in crystals with detergent (Pebay-Peyroula et al. 1995) |
| 2 references |
|---|
| O’Keeffe AH, East JM, and Lee AG (2000) Selectivity in lipid binding to the bacterial outer membrane protein OmpF. Biophys. J.79: 2066-2074. PubMed |
| Pebay-Peyroula E, Garavito RM, Rosenbusch JP, Zulauf M, and Timmins PA (1995) Detergent structure in tetragonal crystals of OmpF porin. Structure 3:1051-1059. PubMed |
