- Protein Classification
- Protein Links
 | out side |
| in side |
| 1kn9 » Type 1 signal peptidase, apo-enzyme | |
|---|---|
| Hydrophobic Thickness | 3.4 ± 1.4 Å |
| Tilt Angle | 80 ± 6° |
| ΔGtransfer | -7.8 kcal/mol |
| Links to 1kn9 | PDB Sum, PDB, SCOP, MSD, OCA, MMDB |
| Topology | out |
| Resolution | 2.4 Å |
| Other PDB entries representing this structure | none |
| Number of TM subunits | 1 |
| Experimental Verification for 1kn9 » Type 1 signal peptidase, apo-enzyme |
|---|
| This is a two-pass TM protein (Uniprot). The isolated periplasmic domain des(2-75) is catalytically active (Paetzel et al. 2002) and associates with lipid bilayers (van Klompenburg et al. 1998, van den Brink-van der Laan et al. 2001). It inserts preferentially into bilayers with decreased headgroup lateral pressure (van den Brink-van der Laan et al. 2004). The following residues of the apo-enzyme are buried in the membrane interior: W300, M301, F303, L314, and L316. This is consistent with the important role of W300 for membrane association (Kim et al. 1995) and with location of N-terminal membrane-anchoring domain. Calculated orientation is similar to that proposed by Paetzel et al. (1998). |
| 6 references |
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| Kim YT, Muramatsu T, Takahashi K. 1995. Identification of Trp300 as an important residue for Escherichia coli leader peptidase activity. Eur J Biochem. 234:358-62. PubMed |
| Paetzel M, Dalbey RE, Strynadka NC. 1998. Crystal structure of a bacterial signal peptidase in complex with a beta-lactam inhibitor. Nature. 396:186-90. PubMed |
| Paetzel M, Karla A, Strynadka NC, Dalbey RE. 2002. Signal peptidases. Chem Rev. 102: 4549-80. PubMed |
| van den Brink-van der Laan E, Dalbey RE, Demel RA, Killian JA, de Kruijff B. 2001. Effect of nonbilayer lipids on membrane binding and insertion of the catalytic domain of leader peptidase. Biochemistry. 40: 9677-9684. PubMed |
| van den Brink-van der Laan E, Killian JA, de Kruijff B. 2004. Nonbilayer lipids affect peripheral and integral membrane proteins via changes in the lateral pressure profile. Biochim Biophys Acta. 1666: 275-88. PubMed |
| van Klompenburg W, Paetzel M, de Jong JM, Dalbey RE, Demel RA, von Heijne G, de Kruijff B. 1998. Phosphatidylethanolamine mediates insertion of the catalytic domain of leader peptidase in membranes. FEBS Lett. 431: 75-79. PubMed |
| Comments on 1kn9 » Type 1 signal peptidase, apo-enzyme |
|---|
| Function - see review by Paetzel et al. (2002). |
