PDB ID or protein name

1kq6 » PX domain of NADH oxidase (p47phox), ligand-free, longer chain

1kq6 » PX domain of NADH oxidase (p47phox), ligand-free, longer chain
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Topology in Endosome membrane
Topologylumenal side
cytoplasmic side
1kq6 » PX domain of NADH oxidase (p47phox), ligand-free, longer chain
Depth 2.4 ± 1.6 Å
Tilt Angle 88 ± 12°
ΔGtransfer -4.6 kcal/mol
Links to 1kq6 PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology cytoplasmic
Resolution 1.1 Å
Other PDB entries of this protein 1gd5, 1o7k
Number of subunits 1
Experimental Verification for 1kq6 » PX domain of NADH oxidase (p47phox), ligand-free, longer chain
This orientation is consistent with association of p47phox to the membrane through residue I65, as observed when this domain is not bound to phosphoinositol lipid (Stahelin et al. 2003). Also see comments for 1o7k.
3 references
Cho W, Stahelin RV. 2005. Membrane-protein interactions in cell signaling and membrane trafficking. Annu Rev Biophys Biomol Struct. 34:119-51. PubMed
Karathanassis D, Stahelin RV, Bravo J, Perisic O, Pacold CM, Cho W, Williams RL. 2002. Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction. EMBO J. 21:5057-68. PubMed
Stahelin RV, Burian A, Bruzik KS, Murray D, Cho W. 2003. Membrane binding mechanisms of the PX domains of NADPH oxidase p40phox and p47phox. J Biol Chem. 278:14469-79. PubMed
Comments on 1kq6 » PX domain of NADH oxidase (p47phox), ligand-free, longer chain
PX domain can be "open" or "closed" for strong association with lipid bilayers depending on interactions with other domains (Karathanassis et al. 2002). This structure may represent the "closed" state, since the hydrophobic beta-turn V18-F19 was buried from water by an additional segment of the polypeptide chain which is missing in 1ok7. Note also different conformations of a loop with exposed W80 residue and different oligomeric states of PX domain in 1ok7 and 1kq6 PDB entries. Both oligomeric states are probably non-native. Therefore, the membrane-bound orientations are calculated for monomers. Subcellular localization of PX domains - see Cho and Stahelin (2005).