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This position was calculated for the complex with bound lipid. Lipid acyl chains were modelled. Calculated hydrocarbon boundary of the lipid bilayer corresponds to the carbonyl groups of the bound lipid. Transfer energy was calculated without contribution from the bound lipid(orientation of the protein without bound lipid is different). Membrane penetration depth of the protein is poorly defined because it strongly depends on the bound lipid conformation (the protein can move away of the hydrophobic boundary).
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