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1py6 » Bacteriorhodopsin, monomer

• Type: 1. Transmembrane (2 classes)
• Class: 1.1. Alpha-helical transmembrane (54 superfamilies)
• Superfamily: 1.1.01. Rhodopsin-like proteins (2 families)
• Family: 1.1.01.01. Ion-translocating microbial rhodopsin (10 proteins) 3.E.1 (TCDB)
• Species: Halobacterium salinarum (3 proteins)
• Localization: Archaebacterial membrane (21 proteins)

1py6 » Bacteriorhodopsin, monomer
Hydrophobic Thickness	31.0 ± 2.5 Å
Tilt Angle	13 ± 6°
ΔGtransfer	-75.9 kcal/mol
Links to 1py6	PDB Sum, SCOP, MSD, OCA, MMDB, Dali
Topology	subunit A (N-terminus extracellular)
Resolution	1.8 Å
Related PDB Sum entries	1bct, 1bha, 1bhb, 1jv6, 1jv7, 1kme, 1pxr, 1pxs, 1q5i, 1q5j, 1s51, 1s52, 1s53, 1s54, 1tn0, 1tn5, 1xji, 3coc, 3cod, 3han, 3hao, 3hap, 3haq, 3har, 3has
Number of TM Secondary Structures	7

1 transmembrane subunit
A - Tilt: 13° - Segments: 1(9-29), 2(43-62), 3(80-98), 4(105-127), 5(134-154), 6(174-196), 7(201-222)

Experimental Verification for 1py6 » Bacteriorhodopsin, monomer
Results are consistent with studies of hydrophobic thickness (Piknova et al. 1993, Dumas et al. 1999) and site-directed spin labeling of bacteriorhodopsin (Altenbach et al. 1990, 1994, Greenhalgh et al. 1991) under conditions when the protein is monomeric.

5 references
Altenbach C, Greenhalgh DA, Khorana HG, Hubbell WL (1994) A collision gradient method to determine the immersion dept of nitroxides in lipid bilayers: application ro spin-labeled mutants of bacteiorhdopsin. Proc. Nat. Acad. Sci. USA91: 1667-1671. PubMed
Altenbach C, Marti T, Khorana HG, Hubbell WL (1990) Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants. Science 248: 1088-1092. PubMed
Dumas F, Lebrun MC, and Tocanne J.F. (1999) Is the protein/lipid hydrophobic matching principle relevant to membrane organization and functions? FEBS Lett.458: 271-277. PubMed
Greenhalgh DA, Altenbach C, Hubbell WL, and Khorana HG (1991) Locations of Arg-82, Asp-85 and Asp-96 in helix C of bacteriorhodopsin relative to the aqueous boundaries. Proc. Natl. Acad. Sci. USA 88: 8626-8630. PubMed
Piknova B, Perochon E, and Tocanne J-F (1993) Hydrophobic mismatch and long-range protein/lipid interactions in bacteriorhodopsin/phosphatidylcholine vesicles. Eur. J. Biochem. 218: 385-396. PubMed

Comments on 1py6 » Bacteriorhodopsin, monomer
Monomeric forms of bacteiorhodopsin are indicated as related PDB entries.

Copyright © 2005- Andrei Lomize, Mikhail Lomize and Irina Pogozheva
University of Michigan, Ann Arbor, MI 48109 USA