PDB ID or protein name

1rh5 » Protein translocase SecY, closed state, mutant

1rh5 » Protein translocase SecY, closed state, mutant
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Topology in Archaebacterial membrane
Topologyextracellular side
cytoplasmic side
1rh5 » Protein translocase SecY, closed state, mutant
Hydrophobic Thickness 29.0 ± 1.1 Å
Tilt Angle 10 ± 0°
ΔGtransfer -93.4 kcal/mol
Links to 1rh5 PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology subunit A (N-terminus cytoplasmic)
Resolution 3.20 Å
Other PDB entries representing this structure none
Number of TM Secondary Structures 12
3 transmembrane subunits
A - Tilt: 8° - Segments: 1(30-43), 2(76-88), 3(110-129), 4(137-158), 5(169-187), 6(209-228), 7(256-276), 8(313-333), 9(379-395), 10(401-411)
B - Tilt: 46° - Segments: 1(36-62)
C - Tilt: 9° - Segments: 1(32-50)
Experimental Verification for 1rh5 » Protein translocase SecY, closed state, mutant
Calculated orientation of hetero-trimeric SecY complex is similar to that in the 2D crystal where this protein forms a dimer of trimers (Breyton et al. 2002). Models of this translocon with bacterial ribosome were generated by MD fitting to EM map (3kcr and 3kc4 PDB entries).
1 reference
Breyton C, Haase W, Rapoport TA, Kuhlbrandt W, and Collinson I (2002) Three-dimensional structure of the bacterial protein-translocation complex SecYEG. Nature 418: 662-665. PubMed