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1wco » Nisin-Lipid II complex in DMSO solution

• Type: 3. Peptides (4 classes)
• Class: 3.4. Peptides of nonregular structure (9 superfamilies)
• Superfamily: 3.4.02. Lantibiotic peptides (class I bacteriocins) (6 families) 1.C.20 (TCDB)
• Family: 3.4.02.03. Nisin (1 protein) 1.C.20 (TCDB)
• Species: Lactococcus lactis (6 proteins)
• Localization: Secreted (681 proteins)

1wco » Nisin-Lipid II complex in DMSO solution
Hydrophobic Thickness or Depth	8.1 ± 1.0 Å
Tilt Angle	90 ± 5°
ΔGtransfer	-8.0 kcal/mol
Links to 1wco	PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology	out
Resolution	NMR
Other PDB entries of this protein	none
Number of subunits	1

Experimental Verification for 1wco » Nisin-Lipid II complex in DMSO solution

Calculated position seems to be consistent with sets of buried residues of nisin in lipid bilayers and micelles (van der Hoven et al. 1996, Bonev et al. 2000, Hsu et al. 2002). However, the comparison is difficult because the peptide is highly flexible, and its conformations in DMSO (1wco) and lipid bilayer may differ (Hsu et al. 2004). Maximal membrane binding affinity is -5.5 kcal/mol, based on the apparent binding constant at low concentration of the peptide (Breukink et al. 2000).

5 references
Bonev BB, Chan WC, Bycroft BW, Roberts GC, Watts A. 2000. Interaction of the lantibiotic nisin with mixed lipid bilayers: a 31P and 2H NMR study. Biochemistry. 39: 11425-33. PubMed
Breukink E, Ganz P, de Kruijff B, Seelig J. 2000. Binding of Nisin Z to bilayer vesicles as determined with isothermal titration calorimetry. Biochemistry. 39: 10247-54. PubMed
Hsu ST, Breukink E, de Kruijff B, Kaptein R, Bonvin AM, van Nuland NA. 2002. Mapping the targeted membrane pore formation mechanism by solution NMR: the nisin Z and lipid II interaction in SDS micelles. Biochemistry. 41: 7670-6. PubMed
Hsu ST, Breukink E, Tischenko E, Lutters MA, de Kruijff B, Kaptein R, Bonvin AM, van Nuland NA. 2004. The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics. Nat Struct Mol Biol. 11: 963-7. PubMed
Van Den Hooven HW, Spronk CA, Van De Kamp M, Konings RN, Hilbers CW, Van De Van FJ. 1996. Surface location and orientation of the lantibiotic nisin bound to membrane-mimicking micelles of dodecylphosphocholine and of sodium dodecylsulphate. Eur J Biochem. 235: 394-403. PubMed

Copyright © 2005-2013 Andrei Lomize, Mikhail Lomize and Irina Pogozheva
University of Michigan, Ann Arbor, MI 48109 USA