PDB ID or protein name

2bcj » PH domain of GPCR kinase 2

2bcj » PH domain of GPCR kinase 2
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Topology in Eukaryotic plasma membrane
Topologyextracellular side
cytoplasmic side
2bcj » PH domain of GPCR kinase 2
Depth 5.7 ± 1.5 Å
Tilt Angle 85 ± 5°
ΔGtransfer -10.4 kcal/mol
Links to 2bcj PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology cytoplasmic
Resolution 3.1 Å
Other PDB entries representing this structure 1omw, 1ym7
Number of subunits 3
Experimental Verification for 2bcj » PH domain of GPCR kinase 2
Consistent with membrane-association mode of the complex proposed by Tesmer et al. (2005) and with locations of two important hydrophobic anchors missing in the crystal structure: an inositol lipid bound to PH domain and prenylated Cys 67(68) in gamma subunit of G-protein.
1 reference
Tesmer VM, Kawano T, Shankaranarayanan A, Kozasa T, Tesmer JJ. 2005. Snapshot of activated G proteins at the membrane: the Galphaq-GRK2-Gbetagamma complex.Science. 310:1686-90. PubMed
Comments on 2bcj » PH domain of GPCR kinase 2
Key membrane-interacting beta-turn in PH domain was modelled using different crystal structure of this kinase (1ym7).