PDB ID or protein name

2gif » Multidrug efflux transporter AcrB, asymmetric

2gif » Multidrug efflux transporter AcrB, asymmetric
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Topology in Bacterial Gram-negative inner membrane
Topologyout side
in side
2gif » Multidrug efflux transporter AcrB, asymmetric
Hydrophobic Thickness 29.0 ± 0.2 Å
Tilt Angle 1 ± 0°
ΔGtransfer -187.5 kcal/mol
Links to 2gif PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology subunit A (N-terminus in)
Resolution 2.9 Å
Other PDB entries representing this structure 2dhh, 2dr6, 2drd, 2hrt, 3aoa, 3aob, 3aoc, 3aod, 3w9h, 4zit, 4ziv, 4ziw, 4zjl, 4zjo, 4zjq, 5jmn, 5yil
Number of TM Secondary Structures 36
3 transmembrane subunits
A - Tilt: 2° - Segments: 1(10-27), 2(340-358), 3(363-386), 4(395-414), 5(438-457), 6(470-493), 7(539-556), 8(873-892), 9(896-918), 10(927-947), 11(971-991), 12(1003-1026)
B - Tilt: 6° - Segments: 1(10-27), 2(340-358), 3(363-383), 4(397-414), 5(438-456), 6(471-493), 7(539-556), 8(874-892), 9(896-918), 10(927-947), 11(971-991), 12(1003-1026)
C - Tilt: 3° - Segments: 1(10-27), 2(340-358), 3(363-386), 4(394-413), 5(439-457), 6(470-492), 7(539-557), 8(873-892), 9(896-918), 10(927-947), 11(972-991), 12(1002-1025)
Comments on 2gif » Multidrug efflux transporter AcrB, asymmetric
Residues 499-515 are ordered in 2gif and 2hrt but disordered in all other structures of AcrB. Three subunits are not identical. Therefore, their tilt angles and some transmembrane segments are slightly different. The substrate is bound to the stronger tilted subunit B.