PDB ID or protein name

2oar » Mechanosensitive channel MscL

2oar » Mechanosensitive channel MscL
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Topology in Bacterial Gram-positive plasma membrane
Topologyout side
in side
2oar » Mechanosensitive channel MscL
Hydrophobic Thickness 36.1 ± 2.2 Å
Tilt Angle 0 ± 0°
ΔGtransfer -142.7 kcal/mol
Links to 2oar PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology subunit A (N-terminus in)
Resolution 3.50 Å
Other PDB entries representing this structure none
Number of TM Secondary Structures 10
5 transmembrane subunits
A - Tilt: 28° - Segments: 1(17-46), 2(69-93)
B - Tilt: 27° - Segments: 1(17-46), 2(69-93)
C - Tilt: 26° - Segments: 1(17-46), 2(69-93)
D - Tilt: 27° - Segments: 1(14-46), 2(69-93)
E - Tilt: 28° - Segments: 1(17-46), 2(69-93)
Experimental Verification for 2oar » Mechanosensitive channel MscL
Calculated membrane core boundaries of MscL seem to be consistent with spin-labeling and fluorescence studies (Perozo et al. 2001, Powl et al. 2003, 2005). However, calculated hydrophobic thickness is much larger than obtained in hydrophobic matching studies.
3 references
Perozo E, Kloda A, Cortes DM, and Martinac B (2001) Site-directed spin-labeling analysis of reconstituted MscL in the closed state. J. Gen. Physiol. 118: 193-205. PubMed
Powl AM, East JM, and Lee AG (2003) Lipid-protein interactions studied by introduction of a tryptophan residue: The mechanosensitive channel MscL. Biochemistry 42: 14306-14317. PubMed
Powl AM, Wright JN, East JM, and Lee AG (2005) Identification of the hydrophobic thickness of a membrane protein using fluorescence spectroscopy: Studies with the mechanosensitive channel MscL. Biochemistry 44: 5713-5721. PubMed