PDB ID or protein name

2rlf » M2 proton channel of Influenza A, closed state, NMR

2rlf » M2 proton channel of Influenza A, closed state, NMR
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Topology in Viral membrane
Topologyout side
in side
2rlf » M2 proton channel of Influenza A, closed state, NMR
Hydrophobic Thickness 34.3 ± 2.0 Å
Tilt Angle 3 ± 1°
ΔGtransfer -67.6 kcal/mol
Links to 2rlf PDB Sum, PDB, SCOP, MSD, OCA, MMDB
Topology subunit A (N-terminus out)
Resolution NMR
Other PDB entries of this protein 1mp6, 1nyj, 2h95, 2kih, 2l0j, 3bkd, 3c9j
Number of TM Secondary Structures 4
4 transmembrane subunits
A - Tilt: 13° - Segments: 1(25-45)
B - Tilt: 14° - Segments: 1(25-45)
C - Tilt: 18° - Segments: 1(25-45)
D - Tilt: 18° - Segments: 1(25-45)
Comments on 2rlf » M2 proton channel of Influenza A, closed state, NMR
This is structure in DHPC micelles. A significant rearrangement of C-terminal surface helix was detected by EPR spin-labeling in membrane: FFK segment probably forms a continuation of TM helix, whereas the C-terminal helices bind to the membrane surface and go in direction opposite to the central channel. This model with classic "rows into grooves" packing of TM helices differs from two crystallographic structures (3bkd and 3c9j; CA atom rmsd of 3.8-3.9 A), and from all solid state NMR models (rmsd >3 A; 2h95 is the closest, with rmsd of 2.3 A). 2kih is S31N mutant.