• Type: Transmembrane (3 classes)
• Class: Alpha-helical polytopic (156 superfamilies)
• Superfamily: Resistance-nodulation-cell division (5 families) 2.A.6 (TCDB) CL0322
• Family: Hydrophobe/amphiphile efflux-1 family (52 proteins) 2.A.6.2 (TCDB) PF00873 IPR004764 PDBsum
• Species: Escherichia coli (636 proteins)
• Localization: Bacterial Gram-negative inner membrane (1251 proteins)

2gif >> Multidrug efflux transporter AcrB, asymmetric 1
Hydrophobic Thickness or Depth	29.0 Å
Tilt Angle	1°
ΔGtransfer	-187.5 kcal/mol
Links to 2gif	PDB Sum, PDB, MSD, MMDB, Encompass
Topology	subunit A (N terminus cytoplasmic side)
Resolution	2.9
Primary PDB represention	2gif
Other PDB entries representing this structure	2dhh (2.8), 2dr6 (3.3), 2drd (3.1), 2hrt (3.0), 3aoa (3.35), 3aob (3.35), 3aoc (3.34), 3aod (3.3), 3w9h (3.05), 4zit (3.3), 4ziv (3.16), 4ziw (3.4), 4zjl (3.47), 4zjo (3.6), 4zjq (3.59), 5jmn (2.5), 5yil (3.0), 6q4n (2.8), 6q4o (2.8), 6q4p (2.8)
Number of TM secondary structures	36
Membranome
Uniprot	ACRB_ECOLI

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Download OPM File: 2gif.pdb

PDB Sum, PDB

Topology in Bacterial Gram-negative inner membrane

	periplasm
	cytoplasmic side

3D view in GLMol | LiteMol | Jmol | iCn3D

Comments: Residues 499-515 are ordered in 2gif and 2hrt but disordered in all other structures of AcrB. Three subunits are not identical. Therefore, their tilt angles and some transmembrane segments are slightly different. The substrate is bound to the stronger tilted subunit B.