1py6 >> Bacteriorhodopsin, monomer
Hydrophobic Thickness or Depth29.6 Å
Tilt Angle24°
ΔGtransfer-59.7 kcal/mol
Links to 1py6PDB Sum, PDB, MSD, MMDB, Encompass
Topologysubunit A (N terminus extracellular side)
Resolution1.8
Primary PDB represention1py6
Other PDB entries representing this structure1jv6 (2.0), 1jv7 (2.25), 1kme (2.0), 1pxr (1.7), 1pxs (2.2), 1q5i (2.3), 1q5j (2.1), 1r2n (NMR), 1r84 (NMR), 1s51 (2.0), 1s52 (2.3), 1s53 (2.0), 1s54 (2.2), 1s8j (2.3), 1s8l (2.3), 1tn0 (2.5), 1tn5 (2.2), 1xji (2.2), 3coc (2.31), 3cod (2.7), 3han (2.75), 3hao (2.49), 3hap (1.6), 3haq (2.3), 3har (1.7), 3has (1.9), 3utv (2.06), 3utw (2.4), 3utx (2.47), 3uty (2.37), 4hwl (2.0), 4hyx (1.99), 4y9h (1.43), 5b34 (2.1), 5b35 (2.35), 5vn9 (2.59)
Number of TM secondary structures7
Membranome
UniprotBACR_HALSA
Topology in Archaebacterial membrane
extracellular side
cytoplasmic side
3D view in GLMol | LiteMol | Jmol | iCn3D

Comments: 1jv6 and 1jv7/1s8j/1s8l are D85S/F219L and D85S mutants (O-like intermediate state). Structures crystallized from bicelles (wild type): 1kme, 1xji, 4hwl, 4hyx, 4y9h, 5b34, 5b35. Crystallized from cubic phase: 5vn9. NMR models: 1r2n, 1r84. Other PDB entries represent different mutants.

Verification: Bacteriorhodopsin, monomer
Results are consistent with studies of hydrophobic thickness (Piknova et al. 1993, Dumas et al. 1999) and site-directed spin labeling of bacteriorhodopsin (Altenbach et al. 1990, 1994, Greenhalgh et al. 1991) under conditions when the protein is monomeric.
Subunits: 1
A - Tilt: 24 - TM segments: 1(8-29),2(43-62),3(80-97),4(105-127),5(131-152),6(177-196),7(201-221)view assembly page
References: 5
Piknova B, Perochon E, and Tocanne J-F (1993) Hydrophobic mismatch and long-range protein/lipid interactions in bacteriorhodopsin/phosphatidylcholine vesicles. Eur. J. Biochem. 218: 385-396. PubMed
Altenbach C, Marti T, Khorana HG, Hubbell WL (1990) Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants. Science 248: 1088-1092. PubMed
Altenbach C, Greenhalgh DA, Khorana HG, Hubbell WL (1994) A collision gradient method to determine the immersion dept of nitroxides in lipid bilayers: application ro spin-labeled mutants of bacteiorhdopsin. Proc. Nat. Acad. Sci. USA91: 1667-1671. PubMed
Dumas F, Lebrun MC, and Tocanne J.F. (1999) Is the protein/lipid hydrophobic matching principle relevant to membrane organization and functions? FEBS Lett.458: 271-277. PubMed
Greenhalgh DA, Altenbach C, Hubbell WL, and Khorana HG (1991) Locations of Arg-82, Asp-85 and Asp-96 in helix C of bacteriorhodopsin relative to the aqueous boundaries. Proc. Natl. Acad. Sci. USA 88: 8626-8630. PubMed