1py6 >> Bacteriorhodopsin, monomer |
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Hydrophobic Thickness or Depth | 29.6 Å |
Tilt Angle | 24° |
ΔGtransfer | -59.7 kcal/mol |
Links to 1py6 | PDB Sum, PDB, MSD, MMDB, Encompass |
Topology | subunit A (N terminus extracellular side) |
Resolution | 1.8 |
Primary PDB represention | 1py6 |
Other PDB entries representing this structure | 1jv6 (2.0), 1jv7 (2.25), 1kme (2.0), 1pxr (1.7), 1pxs (2.2), 1q5i (2.3), 1q5j (2.1), 1r2n (NMR), 1r84 (NMR), 1s51 (2.0), 1s52 (2.3), 1s53 (2.0), 1s54 (2.2), 1s8j (2.3), 1s8l (2.3), 1tn0 (2.5), 1tn5 (2.2), 1xji (2.2), 3coc (2.31), 3cod (2.7), 3han (2.75), 3hao (2.49), 3hap (1.6), 3haq (2.3), 3har (1.7), 3has (1.9), 3utv (2.06), 3utw (2.4), 3utx (2.47), 3uty (2.37), 4hwl (2.0), 4hyx (1.99), 4y9h (1.43), 5b34 (2.1), 5b35 (2.35), 5vn9 (2.59) |
Number of TM secondary structures | 7 |
Membranome | |
Uniprot | BACR_HALSA |
  | extracellular side |
cytoplasmic side |
Comments: 1jv6 and 1jv7/1s8j/1s8l are D85S/F219L and D85S mutants (O-like intermediate state). Structures crystallized from bicelles (wild type): 1kme, 1xji, 4hwl, 4hyx, 4y9h, 5b34, 5b35. Crystallized from cubic phase: 5vn9. NMR models: 1r2n, 1r84. Other PDB entries represent different mutants.
Verification: Bacteriorhodopsin, monomer |
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Results are consistent with studies of hydrophobic thickness (Piknova et al. 1993, Dumas et al. 1999) and site-directed spin labeling of bacteriorhodopsin (Altenbach et al. 1990, 1994, Greenhalgh et al. 1991) under conditions when the protein is monomeric. |
Subunits: 1 |
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A - Tilt: 24 - TM segments: 1(8-29),2(43-62),3(80-97),4(105-127),5(131-152),6(177-196),7(201-221) | view assembly page |
References: 5 |
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Piknova B, Perochon E, and Tocanne J-F (1993) Hydrophobic mismatch and long-range protein/lipid interactions in bacteriorhodopsin/phosphatidylcholine vesicles. Eur. J. Biochem. 218: 385-396. PubMed |
Altenbach C, Marti T, Khorana HG, Hubbell WL (1990) Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants. Science 248: 1088-1092. PubMed |
Altenbach C, Greenhalgh DA, Khorana HG, Hubbell WL (1994) A collision gradient method to determine the immersion dept of nitroxides in lipid bilayers: application ro spin-labeled mutants of bacteiorhdopsin. Proc. Nat. Acad. Sci. USA91: 1667-1671. PubMed |
Dumas F, Lebrun MC, and Tocanne J.F. (1999) Is the protein/lipid hydrophobic matching principle relevant to membrane organization and functions? FEBS Lett.458: 271-277. PubMed |
Greenhalgh DA, Altenbach C, Hubbell WL, and Khorana HG (1991) Locations of Arg-82, Asp-85 and Asp-96 in helix C of bacteriorhodopsin relative to the aqueous boundaries. Proc. Natl. Acad. Sci. USA 88: 8626-8630. PubMed |