1r3j » Potassium channel KcsA
- Type: Transmembrane (3 classes)
- Class: Alpha-helical polytopic (156 superfamilies)
- Superfamily: Ion channel (VIC) superfamily (24 families) CL0030
- Family: KcsA voltage-gated K+ channels (26 proteins) 1.A.1.1 (TCDB) PF07885 IPR013099 PDBsum
- Species: Streptomyces lividans (22 proteins)
- Localization: Bacterial Gram-positive plasma membrane (374 proteins)
1r3j >> Potassium channel KcsA | |
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Hydrophobic Thickness or Depth | 34.8 Å |
Tilt Angle | 0° |
ΔGtransfer | -111.0 kcal/mol |
Links to 1r3j | PDB Sum, PDB, MSD, MMDB, Encompass |
Topology | subunit C (N terminus cytoplasmic side) |
Resolution | 1.9 |
Primary PDB represention | 1r3j |
Other PDB entries representing this structure | 1bl8 (3.2), 1f6g (NMR), 1j95 (2.8), 1jq1 (NMR), 1jq2 (NMR), 1jvm (2.8), 1k4c (2.0), 1k4d (2.3), 1r3i (2.4), 1r3k (2.8), 1r3l (2.41), 1zwi (2.5), 2atk (2.5), 2bob (2.76), 2boc (3.01), 2dwd (2.6), 2dwe (2.5), 2h8p (2.25), 2hg5 (2.75), 2hjf (2.9), 2hvj (2.75), 2hvk (1.9), 2ih1 (2.4), 2ih3 (1.72), 2itc (3.2), 2itd (2.7), 2jk5 (2.4), 2nlj (2.52), 2p7t (2.05), 2qto (3.2), 2w0f (2.4), 3gb7 (2.85), 3hpl (3.2), 3ifx (3.56), 3iga (2.75), 3ogc (3.8), 3or6 (2.7), 3or7 (2.3), 3stl (2.4), 3stz (2.5), 4lbe (2.75), 4lcu (2.75), 4msw (2.06), 5e1a (3.4), 5ebl (2.3), 5ebm (2.5), 5ebw (2.3), 5ec1 (2.75), 5ec2 (2.3), 5j9p (2.85), 5vkh (2.25), 6nfu (2.09), 6nfv (2.13), 6pa0 (2.05), 6w0a (3.24), 6w0f (2.4), 6w0g (2.6), 6w0h (2.6), 6w0i (2.33), 6w0j (2.5) |
Number of TM secondary structures | 8 |
Membranome | ![]() |
Uniprot | IGHG1_MOUSE, IGKC_MOUSE, KCSA_STRLI |
Comments: 2k1e and 2kb2 are models of a water-soluble analogue; 1jq1 and 1jq2 are NMR models of a shorter segment. Several structures of the channel, which were deposited to the PDB without proper biomatrix (1zwi, 2atk, 2jk5, 2w0f and 3hpl), are taken from the PDBTM database.
Verification: Potassium channel KcsA | |
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Locations of hydrophobic boundary planes are consistent with spin-labeling (Perozo et al. 1998, Cross et al. 1999, Cross and Hubbell 2002) and hydrophobic matching (Williamson et al. 2002, 2003) studies of KcsA. Average tilt of TM helices (31°) is consistent with ATR FTIR data (33°)(Le Coutre et al. 1998). |
Subunits: 4 | |||
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C - Tilt: 28 - TM segments: 1(25-50),2(86-111) | |||
D - Tilt: 28 - TM segments: 1(25-50),2(86-111) | |||
H - Tilt: 28 - TM segments: 1(25-50),2(86-111) | |||
J - Tilt: 28 - TM segments: 1(25-50),2(86-111) |
References: 6 | |
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Williamson IM, Alvis SJ, East JM, and Lee AG (2002) Interactions of phospholipids with the potassium channel KcsA. Biophys. J. 83: 2026-2038. PubMed | |
Perozo E, Cortes DM, and Cuello LG (1998) Three-dimensional architecture and gating mechanism of a K+ channel studied by EPR spectroscopy Nature Struct. Biol. 5 (6): 459-469. PubMed | |
Le Coutre J, Kaback HR, Patel CKN, Heginbotham L, and Miller C (1998) Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel. Proc. Natl. Acad. Sci. USA 95: 6114-6117. PubMed | |
Williamson IM, Alvis SJ, East JM, and Lee AG (2003) The potassium channel KcsA and its interaction with the lipid bilayer. Cell Mol. Life Sci. 60: 1581-1590.2. PubMed | |
Gross A, and Hubbell WL (2002) Identification of protein side chains near the membrane-aqueous interface: A site-directed spin labeling study of KcsA. Biochemistry 41: 1123-1128. PubMed | |
Gross A, Columbus L, Hideg K, Altenbach C, and Hubbell WL (1999) Structure of the KcsA potassium channel from Streptomyces lividans: A site-directed spin labeling study of the second transmembrane segment. Biochemistry 38: 10324-10335. PubMed |